1997/10/1/ · B o other sets of 10 eggs each were retrieved after washing, for a total of 40 eggs. When testing the effect of egg collection time, eggs were sampled at about 8 a.m., 1 a.m., and 1 3 p.m. for a total of 120 eggs per sample day. The collection time experimentwas replicated four times, on weeks 52,56,58, and 59 of the production period.